MonoVista CRS - Confocal Raman System
    Making Raman Microscopy Work for You.

Confocal Raman Microscopy or Micro-Spectroscopy is becoming an invaluable tool within Biophysics, Biology and Biochemistry as it can provide more detailed information on the structural composition on cells and biological systems than other more commonly used techniques, such as infra-red absorption spectroscopy.

PI/Acton has over forty years of experience providing high performance CCDs and spectrographs, two of the key components of a high-end Raman system, to the global research community.  We have used our vast experience within this market to create a series of confocal Raman spectrometers that bear the hallmarks of quality and high performance.  The MonoVista series of Confocal Raman systems integrates PI/Acton’s single-stage spectrographs with our extensive range of high-performance CCDs and Olympus Upright BX51 and Olympus Inverted IX71 microscopes to provide a dedicated and flexible research tool. A high-stability base plate ensures long-term stability and repeatable measurements, while our extensive range of detectors ensures that the system has the best sensitivity and detection limits for your particular application. The MonoVista series of confocal raman systems are suitable for a wide range of life-science applications, as confocal Raman spectroscopy can be used as a structural probe for any type of cell or biochemical reaction.  There is a choice of integrated lasers or the systems can be integrated with existing laser systems and a six position filter wheel gives maximum flexibility. The modular design also allows easy reconfiguration for a variety of experiments.

    Features and Benefits:

  • Optically coupled Microscope and/or Macro chamber 
  • High stability base plate, with 5 point mounts  
  • Seamless operation through intuitive yet powerful software interface
  • Integrated lasers available
  • Can support single and multiple existing lasers
  • Imaging spectrometer used
  • Multiple detector options including CCD cameras and InGaAs arrays
  • 6 position filter wheel
  • Dual spectrometer exits
  • Triple grating turret -Over 100 gratings available, including a full line of holographic gratings
XY Mapping 
Raman Microscopy
Confocal Raman Microscopy can be used with practically any sample morphology and provides improved axial and spatial resolution over conventional microscopy. It can be used to highlight a specific portion of a molecule by changing the excitation wavelength and can be used to analyze unstable intermediate species. In addition to these unique advantages, it is non-destructive, requires no special sample preparation and water bands are usually extremely small and subtractable. These numerous benefits allow researchers to perform extremely detailed analysis of cells in their natural state and allow monitoring of the chemistry within individual cells.

Spectral information can be obtained from specific points, as small as 1um, within a sample and spectra obtained from multiple spots can be collected into a three-dimensional data cube, where the x and y dimensions represent spatial co-ordinates and the z dimension stores the spectral information, or a four dimensional data cube that incudes three spatial dimensions plus a spectral dimension. Useful information can be gleamed from the multidimensional data structures by slicing them along any of the spatial or spectral co-ordinates. Thus a comprehensive picture of a sample and its components can be generated from its Raman signatures.

Download: MonoVista CRS Data Sheet