Binning is the process of combining charge from
adjacent pixels in a CCD during readout. This process
is performed prior to digitization in the on-chip
circuitry of the CCD by specialized control of the
serial and parallel registers. The two primary benefits
of binning are improved signal-to-noise ratio (SNR)
and the ability to increase frame rate, albeit at
the expense of reduced spatial resolution.
To understand the process, consider the example
of 2x2 binning shown below. As in normal operation,
charge integrates in individual pixels while the
CCD is exposed to light. During the parallel readout,
the charge from two rows of pixels, rather than
a single row, is shifted into the serial register.
Next, charge is shifted from the serial register,
two pixels at a time, into the summing well. It
then goes to the output amplifier where it is converted
to a voltage before being transferred off-chip for
further amplification and digitization. This procedure
is iterated until the entire array has been read
out. The result is that each readout event from
the summing well contains the collected charge from
four pixels on the CCD. It should be noted that
Photometrics cameras have the ability to perform
binning into any arbitrary MxN binned pixels (superpixels)
through simple software control.
Since both the serial register and
summing well accumulate charge from multiple pixels
during binning, they must have sufficient capacity
to prevent saturation.
In high-performance CCDs, the serial register typically
has a charge capacity double that of the parallel
registers, and the summing well double that of the
serial register. However, the specifications of
the particular CCD being used should be noted and
understood before using the binning technique. This
is especially true when working at high-illumination
levels where saturation could lead to erroneous
data collection.
The primary benefit of binning is higher SNR
due to reduced read noise contributions. CCD read
noise is added during each readout event and in
normal operation, read noise will be added to each
pixel. However, in binning mode, read noise is added
to each superpixel, which has the combined signal
from multiple pixels. In the ideal case, this produces
SNR improvement equal to the binning factors (4x
in the above example). The figure below shows the
effect of 2x2 binning for a four-pixel region. This
example assumes that 10 photoelectrons have been
collected in each pixel and the read noise is 10
electrons. If this region is read out in normal
mode the SNR will be 1:1 and the signal will be
lost in the noise. However, with 2x2 binning, the
SNR becomes 4:1, which is sufficient to observe
this weak signal.
Unlike read noise, dark
current noise is not reduced by binning since
each pixel will contribute dark current noise to
the superpixel. To ensure that dark current noise
does not lower SNR during binning, it is essential
that the CCD be cooled sufficiently to reduce the
dark current noise to a negligible level relative
to the read noise.
One of the most common applications of binning
is spectroscopy. In spectroscopic CCD systems, the
dispersed slit images lie along the CCD columns
(perpendicular to the serial register) and the resultant
images are then binned along the columns. Binning
thus provides marked increases in SNR without any
loss of spectral resolution. Spatial resolution
is lost along the slit axis, but this typically
is not a concern.
Another use of binning is to increase frame rate.
Since the slowest step in the readout sequence is
the digitization of a given pixel, binning can be
used to increase the effective total frame rate
of a given system. Thus, highly binned, low-resolution
images can be obtained when high speed is required
(such as in focusing) and full-frame, high-resolution
images can be obtained when the ultimate resolution
is required. Because this can all be controlled
via software, Photometrics digital cameras are extremely
flexible and can be used in a wide variety of analytical
imaging applications.